Journal: Molecular & Cellular Proteomics : MCP
Article Title: Morphine Regulated Synaptic Networks Revealed by Integrated Proteomics and Network Analysis *
doi: 10.1074/mcp.M115.047977
Figure Lengend Snippet: Network representation of proteins altered by morphine treatment generated using intermediates from a background data set. Genes2FANS (30, 38) was used to connect the up-regulated proteins (green) with the down-regulated proteins (red) from the seed list using a maximum of two intermediates from the background literature-based PPI network. The network contains a total of 99 proteins and 180 interactions. Significant intermediates are shown in orange (z-score > 2.5). Up-regulated proteins (green): CD81, CD81 protein; CLDN11, Claudin-11; GAP43, Neuromodulin; GNAO1, Guanine nucleotide-binding protein G(o) subunit alpha; GNB1, Guanine nucleotide-binding protein G(i)/G(s)/G(t) subunit beta-1; GNB2, Guanine nucleotide-binding protein G(i)/G(s)/G(t) subunit beta-2; PPP3R1, Calcineurin subunit B type 1; RAP1B, Ras-related protein Rap-1b; RPL18A, 60S ribosomal protein L18a; RPS24, 40S ribosomal protein S24; TNX, Thioredoxin. Down-regulated proteins (red): DNAJB5, DnaJ homolog subfamily B member 5; LIMA1, LIM domain and actin-binding protein 1; PAK6, Serine/threonine-protein kinase PAK6; PJA1, E3 ubiquitin-protein ligase Praja-1; TRAF3, TNF receptor-associated factor 3; USP8, Ubiquitin carboxyl-terminal hydrolase 8. Significant intermediates (orange): ADRA2A, Alpha-2A adrenergic receptor; ADRBK1, Beta-adrenergic receptor kinase 1; AKT1, Protein kinase B alpha; CALM3, Calmodulin-3; CASP3, Caspase-3; GNG2, Guanine nucleotide-binding protein G(i)/G(s)/G(o) subunit gamma-2; GRM7, Metabotropic glutamate receptor 7; HD4C4, Histone deacetylase 4; IKBKG, NF-kappa B essential modulator (NEMO); MAP3K14, Mitogen-activated protein kinase kinase kinase 14; MAP3K3, Mitogen-activated protein kinase kinase kinase 3; MAP3K5, Mitogen-activated protein kinase kinase kinase 5; NEDD4, E3 ubiquitin-protein ligase NEDD4; NFKB2, Nuclear factor NF-kappa B p100 subunit; OPRD1, Delta type opioid receptor; OPRk1, Kappa type opioid receptor; OPRL1, Nociceptin receptor; OPRM1, Mu type opioid receptor; RIPK3, Receptor-interacting serine/threonine protein kinase 3; RNF128, E3 ubiquitin protein ligase RNF128; RNF41, E3 ubiquitin protein ligase NRDP1; SPTBN1, Spectrin beta chain, non-erythrocytic 1; TSPAN4, Tetraspanin-4; UBC, Ubiquitin-conjugating enzyme; YWHAZ, 14–3-3 protein zeta/delta.
Article Snippet: Rabbit-anti-PPP3R1/Calcineurin B (Catalog# AP09004PU-N) was from Acris Antibodies GmbH, San Diego, CA.
Techniques: Generated, Binding Assay, Histone Deacetylase Assay
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